Review



px459 background  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Addgene inc px459 background
    Px459 Background, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2928 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+plasmid+background/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pmc09637994__mmc4-269-22-28
    Average 96 stars, based on 2928 article reviews
    px459 background - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: YY1 binding is a gene-intrinsic barrier to Xist-mediated gene silencing
    Article Snippet: .. Single guide RNAs for introducing double strand breaks at the C-terminal region of Yy1 were designed using the CRISPOR online tool ( ) and cloned into the pX459 plasmid background (Addgene #62988) using the Zhang lab digestion-ligation protocol (Broad Institute). .. The homology vector was cloned by Gibson assembly (Gibson Assembly Master Mix kit, NEB) into a restriction-enzyme digested pCAG backbone plasmid.

    Article Title: YY1 binding is a gene-intrinsic barrier to Xist-mediated gene silencing
    Article Snippet: .. Single guide RNAs for introducing double-strand breaks at the C-terminal region of Yy1 were designed using the CRISPOR online tool (Concordet and Haeussler, ) and cloned into the pX459 plasmid background (Addgene #62988) using the Zhang lab digestion-ligation protocol (Broad Institute). .. The homology vector was cloned by Gibson assembly (Gibson Assembly Master Mix kit, NEB) using primers given in Dataset into a restriction-enzyme digested pCAG backbone plasmid.

    Article Title: YY1 binding is a gene-intrinsic barrier to Xist-mediated gene silencing.
    Article Snippet: .. Single guide RNAs for introducing double-strand breaks at the C-terminal region of Yy1 were designed using the CRISPOR online tool (Concordet and Haeussler, 2018) and cloned into the pX459 plasmid background (Addgene #62988) using the Zhang lab digestion-ligation protocol (Broad Institute). .. The homology vector was cloned by Gibson assembly (Gibson Assembly Master Mix kit, NEB) using primers given in Dataset EV3 into a restriction-enzyme digested pCAG backbone plasmid.

    Plasmid Preparation:

    Article Title: YY1 binding is a gene-intrinsic barrier to Xist-mediated gene silencing
    Article Snippet: .. Single guide RNAs for introducing double strand breaks at the C-terminal region of Yy1 were designed using the CRISPOR online tool ( ) and cloned into the pX459 plasmid background (Addgene #62988) using the Zhang lab digestion-ligation protocol (Broad Institute). .. The homology vector was cloned by Gibson assembly (Gibson Assembly Master Mix kit, NEB) into a restriction-enzyme digested pCAG backbone plasmid.

    Article Title: YY1 binding is a gene-intrinsic barrier to Xist-mediated gene silencing
    Article Snippet: .. Single guide RNAs for introducing double-strand breaks at the C-terminal region of Yy1 were designed using the CRISPOR online tool (Concordet and Haeussler, ) and cloned into the pX459 plasmid background (Addgene #62988) using the Zhang lab digestion-ligation protocol (Broad Institute). .. The homology vector was cloned by Gibson assembly (Gibson Assembly Master Mix kit, NEB) using primers given in Dataset into a restriction-enzyme digested pCAG backbone plasmid.

    Article Title: YY1 binding is a gene-intrinsic barrier to Xist-mediated gene silencing.
    Article Snippet: .. Single guide RNAs for introducing double-strand breaks at the C-terminal region of Yy1 were designed using the CRISPOR online tool (Concordet and Haeussler, 2018) and cloned into the pX459 plasmid background (Addgene #62988) using the Zhang lab digestion-ligation protocol (Broad Institute). .. The homology vector was cloned by Gibson assembly (Gibson Assembly Master Mix kit, NEB) using primers given in Dataset EV3 into a restriction-enzyme digested pCAG backbone plasmid.



    Similar Products

    px459 background  (Addgene inc)
    96
    Addgene inc px459 background
    Px459 Background, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+plasmid+background/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pmc09637994__mmc4-269-22-28
    Average 96 stars, based on 1 article reviews
    px459 background - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    px459 plasmid background  (Addgene inc)
    96
    Addgene inc px459 plasmid background
    Px459 Plasmid Background, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+plasmid+background/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm38654121-123-28-31
    Average 96 stars, based on 1 article reviews
    px459 plasmid background - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results